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RNA quality is one of the major factors affecting the outcome of a microarray experiment. The Agilent 2100 bioanalyzer is a microfluidics-based platfom for the analysis of DNA, RNA, and proteins.
It has become a viable alternative to gel electrophoresis techniques. It delivers fast, automated, high quality digital data using a minimal amount of template.
Agilent Bioanalyzer Analysis
Options:
Chip Type |
Volume Needed |
Concentration Required |
# Samples per Chip |
Price per chip |
RNA Pico |
2-4ul |
1-5ng/ul |
11 |
$50.00 |
RNA Nano |
2-4ul |
50-500ng/ul |
12 |
$50.00 |
DNA 1000 |
2-4ul |
0.1-50ng/ul |
12 |
$50.00 |
Submissions:
-We recommend samples be submitted in 200ul PCR strip tubes, with each tube labeled individually with sample name and PI’s initials.
-Samples should be submitted in water or TE buffer. Contaminants of any kind, including salts, organic compounds, metals, or dust, will interfere with the results.
-Submission form must be completed and dropped off with samples ( submission form) OR sent by email (see contacts). If you are submitting the form by email, please have your PI send an email confirming the account number.
-Please contact the lab before bringing your samples so we can ensure that they are placed immediately into the -80C freezer.
-Samples must be brought to the lab on ice or dry ice.
-Please indicate on the submission form if you would like any remaining sample back, and please note that the sample will be denatured.
Results:
-The Bioanalyzer program will generate data concerning the integrity of your samples. Your results will include electropherograms and gel-like images, as well as a ribosomal ratio.
-The results will also show a concentration, however, we recommend using the Nanodrop for the most accurate concentration results.
-Results include an RNA Integrity Number (RIN). Definition and explanation can be found here: RIN_Mueller, Lightfoot & Schroeder reference[1].
FAQs:
Q: How long will it take to get my results?
A: The normal turnaround time is 1-2 business days.
Q: What is the lowest RIN acceptable for use with Affymetrix?
A: We recommend that the RIN be at least 7. However, we have obtained acceptable results with RINs as low as 6.7.
Q: I have less than 12 samples (for nano) or 11 samples (for pico). Do I have to pay for a whole chip?
A: We are willing to store your samples for a short period of time at -80C. Should another PI submit less than the maximum number of samples during this time, we will run your samples on the same chip and split the cost.
Contacts:
-Please contact Mary Majewski (mmajewsk@uiuc.edu), Carole Wilson ( cawilson@uiuc.edu), or Tanya Akraiko (akraiko@uiuc.edu) with any additional questions.
-Lab address/shipping information:
University of Illinois
356 Edward R. Madigan Laboratory
1201 W. Gregory Dr.
Urbana, IL 61801
-Phone number:
217-244-3929
For further information and service fees, contact: Carole Wilson or Mark Band in the Functional Genomics Lab at (217)244-3929
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