Maui Hybridization Mixer

The W.M. Keck Center has installed a
Maui hybridization mixer for use with
printed microarrays on glass slides. The
Maui mixer uses special cover slips or
mixing chambers which are applied to
the microarray slide via an adhesive
gasket; forming a uniform 25 µm high,
sealed hybridization chamber. Mixing of
the hybridization buffer occurs by

Please contact Dr. Mark Band
(markband@uiuc.edu) for details on
availability and use of this protocol.

inflation of small air bladders at either
end of the chambers. Mixing improves
hybridization signal, especially of low
expression probes while background
remains the same. Higher signal due to
mixing enables the use of lower starting
amounts of nucleic acids or shorter
hybridization times.