Mouse Microarrays, W. M. Keck Center, Biotechnology Center UofI

NIA15K Mouse Microarrays

The Keck Center has received a 15,000 clone set derived from mouse embryonic and ovary cDNA libraries from the National Institute of Aging (NIA15K). The microarrays developed from this library are available to all researchers from both the Champaign-Urbana and Chicago campuses of the University of Illinois as well as outside researchers.

The NIA15K array is available on Corning GAPS II glass slides. The array is spotted with 48 pins in a 4 x 12 pattern. Endogenous mouse control spots and exogenous plant genes as negative or spiking controls are printed on each grid to allow for normalization and determination of signal variation across the slides. Clone ID and sequence information about the NIA15K set is available and can be queried or downloaded from the National Institute of Aging website: http://lgsun.grc.nia.nih.gov/cDNA/15k.html

The array file is in Axon GAL file format.
Reminder: Results from these and other microarrays should be confirmed by alternative methods such as Real-Time PCR.

Download array file: NIA GAL file

Download array slide image showing position of array on the slide.

The control set printed on this array is presented below with gene name and GenBank accession number:

Mouse controls
NIA clone	Accession#	Symbol	Gene Name
H3002D02	AA409871	Actx	melanoma X-actin
H3023D06	AW536085	Pgk1	phosphoglycerate kinase 1
H3152G02	AW557425	Hprt	hypoxanthine guanine phosphoribosyl transferase
H3014C06	AW541488	B2m	beta-2 microglobulin
H3101C10	AU043933	Gapd	glyceraldehyde-3-phosphate dehydrogenase

Spiking Controls (Arabidopsis Thaliana) RT-PCR from cDNA
X56062 ATCABI	Cab mRNA for photosystem I chlorophyll A/B-binding protein.
X14212 ATRCA	Arabidopsis thaliana mRNA for rubisco activase
U91966 ATrbcl	Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene

Exogenous Controls (soy)
AI495218	GmRBS1 Soy Rubisco small chain 1
	GmCAB Soy Chlorophyll ab binding protein Gm-cl037-2792

AJ239127	GmMSG Soy MSG

Spiking controls (Arabidopsis) (200 ng/ul) Strategene catalogue # 252005

CAB   X56062
RCA   X14212
rbcL   U91966

Primers used to amplify Arabidopsis cDNA or Soy clones:
	ATRCA F	5'-GATTCGCACAGAGCAACAAG-3'
	R	3'-GGTACCTCTGACGGATAAGC-3'
	ATCAB1 F	5'-TCGAACTCGCTTATGAGCTG-3'
	R	5'-GGTCCTTCGAGTATCCAAGA-3'
	ATrbcl F	5'-CCGAGTAACTCCTCAACCTG-3'
	R	3'-AACGGTCTCTCCAACGCATA-3'
	GmRBS1 F	5'-CTTGGCGAACGTTGTCGAATC-3'
	R	5'-AGTTACCACTGTCAACCGTGC-3'
	GmCAB1 F	5'-AGGTCCTCCTGGATAAGCTGG-3'
	R	3'-CAAGCAAGGATCAGACAGACC-3'
	GmMSG F	5'-ACGCACGGCAATTTAAAGCA-3'
	R	5'-TGTAGCCCCTCATCATATCCA-3'

Post Printing Processing
The NIA15K slides that you have received from the UIUCFunctional Genomics lab have already been fixed by baking at 80C and treated in an SDS wash.The printed and treated face of the slide is facing you when the short notched side is at the top with the notch on the upper right corner.

Functional Genomics Unit
Mark Band, Ph.D - Director
356 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801
Phone: (217) 244-3930 FAX: (217) 265-5066 Email: markband@uiuc.edu

Last edited: 22 Nov 2004