Real Time PCR, Functional Genomics Unit

Real Time PCR	The Taqman ABI 7900 real time PCR machine is operational and available for use by all university researchers. The use of low volume reactions and 384 well plates conserves both reagents and budget. In addition, our machine is equipped with an autoloading robot allowing 24 hour use of the machine. Real time PCR is one of the most sensitive and accurate assays for RNA expression analysis allele discrimination or DNA quantification. At present all users are trained for sample setup and analysis by Functional Genomics Lab staff. Users prepare their samples independently and bring in their PCR plates ready to run. The Primer Express and Sequence Analysis software is available to be used in the Functional Genomics Lab..

For University of Illinois researchers there is a $35.00 fee/plate. This covers only maintenance and service contracts. Please provide an account number when making an appointment to use the machine. Plasticware and reagents required for Real-Time PCR analysis. We strongly suggest reading the following technical bulletins published by Applied Biosystems explaining the principles of Real-Time PCR before planning your assays. Designing MGB Probes and Primers Essentials of realtime PCR Guide to relative quantitation Rapid Assay developement guidelines Real time vs. traditional PCR User bulletin 2 For more information contact Dr. Mark Band at the Functional Genomics Lab. Tel: (217)244-3930 E-mail: markband@uiuc.edu
Functional Genomics Unit Mark Band, Ph.D - Director 356 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801 Phone: (217) 244-3930 FAX: (217) 265-5066 Email: markband@uiuc.edu
Last edited: 22 Nov 2004