Sanger Sequencing Services
The High-Throughput Sequencing team provides a vast array of services for high-throughput sequencing projects, (greater than 12 96-well plates in a three month period) and customers are allowed to submit samples starting at any stage in the Sanger sequencing process. For the Sanger dideoxy-terminator sequencing, we can take a project from the beginning stages of culturing the bacteria, up to full ready to load plates. The plasmid preps used for sequencing are purified using the Qiagen 9600 BioRobot. Conditions for each project are optimized to produce the best sequencing data. The Robbins Hydra is utilized for precise reaction set-ups. Cycling conditions are optimized on the BioRad/MJ Research DNA tetrads. Big Dye terminator chemistry is used for Sanger sequencing reactions and all sequencing is performed on the ABI 3730XL capillary systems. All 384- and 96-well format plates are labeled with a barcode, and a high throughput laboratory information management system (HTLIMS) is used to track sample flow from plate submission through sequencing.
Please contact Alvaro Hernandez, Director of DNA Services ( aghernan@illinois.edu ) at 217-244-3480 or Chris Wright, Assistant Director of DNA Services ( clwright@illinois.edu) at 217-333-4372 to discuss ways the staff can be of assistance in achieving your project goals and/or to provide a quote for your project.
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High-Throughput Sequencing and Genotyping Unit
Director: Alvaro Hernandez, Ph.D.
340 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801
Phone: (217) 244-3480 FAX: (217) 265-5066 Email: aghernan@illinois.edu
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| Last edited: 16 July 09 |
