Roche 454 GS-FLX system: Ultra-high-throughput sequencing for genomic DNA, environmental samples (metagenomes), PCR amplicons, amplified tag fragments (microRNA, ChiP-derived, methlyation enriched, captured sequences, SAGE, etc.), BAC clones or pools, and cDNA.

• RNA extraction per tissue: $200
• cDNA Synthesis and normalization: $5,000
• Amplicon quantity and quality measurement: $200
• Tags are $600 each

• 454-library preparation (nebulization and adaptor ligation): $700
• Titration (with subsequent bulk run): $2,500

• 70x75 PTP run using LR70
Full plate: $8,400
1/2 plate: $4,685
1/4 plate: $2,728
1/8 plate: $2,086
1/16 plate: $1,653

• 70x75 PTP run using SR70
Full plate: $6,670
1/2 plate: $3,820
1/4 plate: $2,295
1/8 plate: $1,870
1/16 plate: $1,545

• 25x75 PTP run using LR25
Full: $4,200

High Throughput DNA Sequencing (HTS): On-campus service fees based upon sequencing of 12 or more 96-well plates within a 3-month period, excepting certain HTS Full Service projects (eg: shotgun libraries) which will allow 6 or more 96-well plates within a 3-month period. 

 No more than 2 primers are allowed per project for HTS Full Service and HTS No Prep projects.  For projects that do not meet the minimum plate requirements, or those with more than 2 primers, please consult the DNA Core Sequencing pages for Core and Medium Throughput services and prices.

 HTS Full Service:  Culture, prep, reactions, clean-up, loading on 3730xl, data pickup site:

ØRequirement: ≥ 12 96-well plates in 3 months(except shotgun libraries); 2 primer maximum

Full service HTS is through one of two options for template preparation:
1. Heat lysis DNA template preparation

  Ø   $1.50/well ($144/96-well plate) for first primer.

  Ø $1.30/well ($124.80/96-well plate) for second primer on same template.

2. Filter plate DNA template preparation

  Ø   $2.25/well ($216/96-well plate) for first primer.

  Ø $1.30/well ($124.80/96-well plate) for second primer on same template.

Overall, the project sequencing success rate is nearly equivalent for both
procedures, but the sequence read lengths average approximately up to 100bp longer with the
filter prepared template versus heat lysis template preparation options.

HTS No Prep:  Reactions, clean-up, loading on 3730xl, data pickup site.

Ø      Requirements: > 12 96-well plates in 3 months; 2 primer maximum.

Ø      $1.30/well ($124.80/96-well plate) for first primer, same price for second primer if desired.

HTS-Bac end:

Ø    Full service BAC-end is $2.50 per sample ($240/96-well plate )

ØSequencing from a purified BAC(no prep) is $1.60 per sample ($153.60/96-well plate)

 HTS Ready-to-Load (RTL) plus Clean-up:  Clean up, loading on 3730xl, data pickup site.

Ø      Requirements: > 12 96-well plates in 3 months.

Ø      $80.00/96-well plate.

 HTS RTL: Loading on 3730xl, data pickup site.

Ø      Requirements: > 12 96-well plates in 3 months.

Ø     $75.00/96-well plate).

 Colony Picking:  Uses Genetix Qpix II robotic colony picker.

Ø      $150 set-up fee per library plus $0.10 per clone.

Ø      $8.00 for duplicate plates (96-well or 384-well).

 Rearraying/Reracking:  Uses Genetix Qpix II robotic rearrayer.

Ø      Creates a new subset of plates from previously sequenced plates (useful for 3’ sequencing of plasmid clones or preparation for template amplification for microarray printing).  The investigator will be provided with a detailed printout of clone ID numbers for each well.  Rearray fidelity is confirmed through sequencing.

Ø      $450 set-up fee plus $0.60 per clone. Duplicate plates included at no charge

 This is an abridged list of our service prices and subject to change at any time.  For further information contact Chris Wright (217-333-4372, clwright@uiuc.edu).

 The full fee applies to all wells left empty on a plate.  If the percentage of successful reactions on a plate is below 80% (defined as 200 high quality bases) due to a problem deemed to be a technical issue in the Keck Center, the price per plate will be adjusted proportionally downward.  Sequencing success rates will be calculated for each plate and those with success rates of 30% and above will be billed.  If the sequencing project is aimed at sequence confirmation (e.g. rearrayed plates, 3’ end sequencing), then plates with success rates below 50% will be reprocessed.  The investigator will be billed base on the combined success rates of the original and the repeated plates. 

Facility users other than State of Illinois institutions are subject to a 25.3% indirect cost rate for federally funded projects. For off-campus projects a contract must be negotiated through University of Illinois Grants and Contracts.  For further information contact Dr. Mark A. Mikel (217-244-0144; mmikel@uiuc.edu), Associate Director of the Roy J. Carver Biotechnology Center.