Roche 454 GS-FLX+: Ultra-high-throughput sequencing for genomic DNA, cDNA, environmental samples (metagenomes), PCR amplicons, amplified tag fragments (microRNA, ChIP-derived, methlyation enriched, captured sequences, SAGE, etc.), BAC clones or pools, and more. For descriptions and guidelines for Roche/454 sample submission, please visit our Roche/454 Sequencing Page.

Roche GS FLX+ Library Prep Services:

• cDNA synthesis and normalization: $1,425. $985 for additional libraries prepared at the same time.
• Amplicon (PCR products) quantity and quality measurement(for up to twelve samples): $205.

• Roche GS FLX+ Titanium library preparation (nebulization and adaptor ligation): $410 per library. Up to 12 MIDs (tags) can be included at no charge if requested.

• Roche GS FLX+ Paired End (3kb, 8kb, or 20kb) library preparation: $1085 for the first library, $820 for any additional library of the same size prepared from the same sample. MIDs (tags) can be included at no charge if requested, but may significantly decrease true percent paired-end reads.

Roche GS FLX+ Sequencing Services including Amplicons:

• Titration (Strongly recommended for projects sequencing ½ bulk PTP or more): $1430

Illumina HiSeq2000 system: Ultra high-throughput sequencing of mRNA, genomic DNA, ChIP DNA and small RNAs.

Sample Preparation Services :

• RNASeq (bacterial): $405
• Small RNA libraries: $430
• gDNASeq, RNASeq (eukaryotic): $340 per library
• Mate-Pairs (3kb and 8kb jumps): $825 per library

Sequencing Services using Version 3 clusters and sequencing kits:

• Single read sequencing, 50nt reads: $1,310 per lane
• Single read sequencing, 100nt reads: $1,560 per lane
* Paired-end sequencing, 100nt reads: $2,640 per lane

High Throughput Sanger DNA Sequencing (HTS) : On-campus service fees based upon sequencing of 50 or more 96-well plates within a 2-month period. No more than 2 primers are allowed per project for HTS Full Service and HTS No Prep projects.  For projects that do not meet the minimum plate requirements, or those with more than 2 primers, please consult the DNA Core Sequencing pages for Core and Medium Throughput services and prices. For descriptions and guidelines for Sanger sample submission, please visit our Sanger Sequencing Services Page.

  HTS Full Service:  Culture, prep, reactions, clean-up, loading on 3730xl, data pickup site:
    Filter plate DNA template preparation

• $235.00/96-well plate for first primer.
• $170.00/96-well plate for second primer on same template.

  HTS No Prep:  Reactions, clean-up, loading on 3730xl, data pickup site.

• $140.00/96-well plate for first primer, same price for second primer if desired.

  HTS BAC-end Sequencing:

• Full service BAC-end Sequencing is $207.60/96-well plate
• Sequencing from a purified BAC(no prep) or second primer is $222.00/96-well plate

  HTS Ready-to-Load (RTL) plus Clean-up:  Clean up, loading on 3730xl, data pickup site.

• $140.00/96-well plate.

  HTS RTL: Loading on 3730xl, data pickup site.

• $90.00/96-well plate  

 This is an abridged list of our service prices and subject to change at any time.  For further information please contact Dr. Alvaro Hernandez, Director of DNA Services ( aghernan@illinois.edu ) at 217-244-3480 or Chris Wright, Assistant Director of DNA Services ( clwright@illinois.edu ) at 217-333-4372 to discuss ways the staff can be of assistance in achieving your project goals or to receive a quote for your project.  

The full fee applies to all wells left empty on a plate.  If the percentage of successful reactions on a plate is below 80% (defined as 200 high quality bases) due to a problem deemed to be a technical issue in the Keck Center, the price per plate will be adjusted proportionally downward.  Sequencing success rates will be calculated for each plate and those with success rates of 30% and above will be billed.  The investigator will be billed base on the combined success rates of the original and the repeated plates. 

Facility users other than State of Illinois institutions are subject to a 25.3% indirect cost rate for federally funded projects. For off-campus projects a contract must be negotiated through University of Illinois Grants and Contracts.  For further information contact Dr. Mark A. Mikel (217-244-0144; mmikel@illinois.edu ), Associate Director of the Roy J. Carver Biotechnology Center.