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Lorie Rickords, Senior Research Specialist

Dot graphic Custom Oligonucleotide Synthesis

Custom Oligonucleotide Synthesis is carried out on two 394 Synthesizers from ABI/Perkin Elmer. These instruments are excellent for large scale (40 nmol - 10 umol) and long (up to 150 bps) oligonucleotide synthesis, specially modified oligonucleotides, and allow for the option of additional purification procedures required for highly sensitive research applications.

Four synthesis scales are available in Custom productions: the 40 nmol, .2 umol, 1 umol, and 10 umol. All synthesis scale samples are processed as Crude. The 40 nmol and .2 umol scales can be purified using OPC or PAGE purification. We now offer polypak purification for 1umol samples. For description of Crude vs. Purified oligos, please check out our Crude Oligo page. Oligos up to 150 bases in length may be synthesized. Standard degenerate bases are accepted at no extra charge and must follow IUB nomenclature. Non standard degenerate bases may be made at an extra charge of $10.00 per mix. A number of special bases and oligo modifications are available. Please check out our special modifications page which lists many of our more common requests. Prices vary for individual projects, so please call for more information.

40 nmol scale Avg. Min. Yield
(ug)
Avg. Turn 
Around Time
.2 umol scale Avg. Min. Yield
(ug)
Avg. Turn 
Around Time
< 50 bases available 132 24 hrs available 660 24 hrs
51-80 bases available 132 24 hrs available 660 24 hrs
81+ bases N/A - - available 660 24 hrs
OPC Purif. available 66 24-48 hrs available 330 48-72 hrs
PAGE Purif. available 30 48-72 hrs available 120 72-96 hrs
  1 umol scale Avg. Min. Yield
(mg)
Avg. Turn 
Around Time
10 umol scale Avg. Min. Yield
(mg)
Avg. Turn 
Around Time
< 20 bases available 3.3 96 hrs available 33 96 hrs
21-50 bases available 3.3 96 hrs N/A -  

 

Custom oligos cannot be ordered over the net at this time.  A downloaded form may be faxed to us at 217-265-5066, emailed (see addressed below), or you may fill out a form at our office in room 329/340 ERML.

Download Custom Oligo Order Form (Word format or pdf format)

(If you are an off campus customer,please complete the off-campus user form)

CUSTOM OLIGONUCLEOTIDE SYNTHESIS ACADEMIC PRICES
*** For non-academic prices please inquire***

View Standard Primers | IUB Formats & Custom Modifications

Disclaimer: Our products are guaranteed to meet or exceed our published specifications for quality and yield when used under normal conditions in your laboratory. If our product is defective or you are dissatisfied with our product, please inform us within 60 days of synthesis. No other warranties are expressed or implied, including, but not limited to, the implied warranty of fitness for a particular purpose. We shall not be liable for consequential damages.

Oligonucleotide Synthesis Services
Research Specialist: Virginia Lukas - vlukas@illinois.edu
Senior Research Specialist: Lorie Hetrick- lhetrick@illinois.edu
Research Specialist: Erica Hoagland - ehoaglan@illinois.edu
329 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801
Phone: (217) 244-1007     FAX: (217) 265-5066 

Last edited: 12 August 2008