|
Custom Oligonucleotide Synthesis
Custom Oligonucleotide Synthesis
is carried out on two 394 Synthesizers from ABI/Perkin Elmer. These
instruments are excellent for large scale (40 nmol - 10 umol) and long (up
to 150 bps) oligonucleotide synthesis, as well as, specially modified oligonucleotides,
and allow for the option of additional purification procedures required
for highly sensitive research applications.
Synthesis scales available in Custom productions include 40 nmol, .2
umol, 1 umol, and 10 umol. All synthesis scale samples may be processed as Crude.
The 40 nmol scale can be purified using OPC or PAGE gel options. The 0.2 umol scale may be purified via PolyPak or PAGE gel options.
We now offer PolyPak and PAGE gel purification for 1umol samples. For a description of Crude vs. Purified oligos, please check out our Crude
Oligo page. Oligos up to 150 bases in length may be synthesized. Standard degenerate bases
are accepted at no extra charge and must follow IUB nomenclature. Non standard
degenerate bases may be made at an extra charge of $10.00 per mix. A number of
special bases and oligo modifications are available. Please check out our special
modifications page which lists many of our more common requests. Prices vary
for individual projects, so please call for more information.
Service Fee Available.
|
40 nmol scale
|
Avg. Min. Yield
(ug)
|
Avg. Turn
Around Time
|
.2 umol scale
|
Avg. Min. Yield
(ug)
|
Avg. Turn
Around Time
|
| < 50 bases |
available |
132 |
24 hrs |
available |
660 |
24 hrs |
| 51-80 bases |
available |
132 |
24 hrs |
available |
660 |
24 hrs |
| 81+ bases |
N/A |
- |
- |
available |
660 |
24 hrs |
| OPC Purif. |
available |
66 |
24-48 hrs |
available |
330 |
48-72 hrs |
| PAGE Purif. |
available |
30 |
48-72 hrs |
available |
120 |
72-96 hrs |
| |
1 umol scale |
Avg. Min. Yield
(mg) |
Avg. Turn
Around Time |
10 umol scale |
Avg. Min. Yield
(mg) |
Avg. Turn
Around Time |
| < 20 bases |
available |
3.3 |
96 hrs |
available |
33 |
96 hrs |
| 21-50 bases |
available |
3.3 |
96 hrs |
Available |
- |
|
Custom oligos cannot be ordered over the net at this time. A downloaded
form may be faxed to us at 217-265-5066, emailed (see addressed below),
or you may fill out a form at our office in room 329/340 ERML.
Download Custom
Oligo Order Form (Word format or pdf format)
(If you are an off campus customer,please complete the off-campus user form)
CUSTOM OLIGONUCLEOTIDE SYNTHESIS ACADEMIC PRICES
***
For non-academic prices please inquire***
|