Sample analysis using the new LIZ500 5-dye system.
Fragment Analysis
DNA fragment analysis is performed on the ABI Prism 3730xl,
a capillary electrophoresis platform that provides accurate
size calling and high reproducibility. Our facility can analyze
fragments from 50-1000bp in size from many different
genotyping applications.
Size Standards
Several size standards are available to analze fragments with 3-dye or 4-dye multiplexing. Size standards are provided by our facility and do not need to be purchased separately. Please call if you would like to use a custom size standard.
| Size Standard |
Size Range (bp)
|
Blue Dye Label |
Green Dye Label |
Yellow Dye Label |
Red Dye Label |
|
LIZ120
|
50-120bp
|
dR110
|
dR6G
|
dTAMRA
|
dROX
|
|
ROX400
|
50-400
bp
|
6-FAM
|
HEX
|
NED
|
|
ROX500 |
50-500bp |
6-FAM |
HEX |
NED |
|
LIZ500 |
50-500bp |
6-FAM |
VIC |
NED |
PET |
LIZ600 |
50-600bp |
6-FAM |
VIC |
NED |
PET |
|
ROX1000
|
50-1000bp
|
6-FAM
|
HEX
|
NED
|
- |
| LIZ1200 |
50-120bp |
6-FAM |
VIC |
NED |
PET |
Pricing and Submission Information
Pricing is dependent on the expected throughput of your project. Fees below include size standard, electrophoresis on the ABI 3730xl and access to GeneMapper analysis software. For each new customer, we provide one free 96-well test plate of serial dilutions to determine optimal DNA concentrations.
Project Size |
Univeristy of Illinois
Urbana-Champaign |
Other Illnois Universities |
Out-of-state universities, government facilities, private companies or international laboratories |
Low-Throughput
orders of 95 samples or less |
$3.00 ea |
$3.30 ea |
$3.76 ea |
Medium/High-Throughput
orders of 96-well plates
|
$105.00/plate |
$115.50/plate |
$157.00/plate |
For questions about choosing size standards and primer dye labels, for order submission and obtaining access to software
for analysis, please check out the Fragment Analysis FAQ
or contact Core Staff at dna-seq@illinois.edu or 217-333-9520
|
