Surface Antigen Quantification

By using graduated solutions of microbeads with known amounts of fluorophore. Comparisons can be made to populations with identical fluorophores bound to antigens. Quantification can be done due to the fact that mean fluorescent intesity of a cell population labeled with a fluorophore conjugated to antibodies is linearly related to the mean number of binding sites per cell.

 

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Flow Cytometry Facility Barbara Pilas, Ph.D - Director 231 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801 Phone: (217) 244-0559     FAX: (217) 244-0446      Email: http://uiuc.edu/

Last edited:09 June 2008