Satellite Facilities

The Flow Cytometry Facility has five satellite laboratories that provide trained users around-the-clock access to flow cytometry instrumentation.

• Room 221 of the Edward R. Madigan Laboratory (221 ERML)

The laboratory is equipped with a Typhoon^TM Molecular Dynamics phosphor/fluorescence imager. This is a versatile multimode scanner, capable of phosphorescence, 4 color fluorescence, and chemiluminescence detection in gels.



Typhoon^TM Molecular Dynamics Phosphor/Fluorescence Imager


The laboratory is also equipped with a Bio-Rad Bio-Plex Cytometric Bead Analyzer. This is an easy to operate machine for use with cytokine (up to 50 human and 32 mouse) and phosphoprotein (up to 26) assays in 96 well plates.


Bio-Rad Bio-Plex Cytometric Bead Analyzer

• Room 1404 of the Veterinary Medicine Basic Sciences Building (1404 VMBS)

The VP facility is equipped with a Coulter XL 3 color flow cytometry analyzer. After attending a 4 hour training course, users are given access to the labs on a 24 hours per day, 7 days per week basis.

• Rooms 315 & 348 Burrill Hall
  

These laboratories are equipped with Coulter XL-MCL, 4 color flow cytometry analyzer which are suitable for most routine flow cytometric analyses. These are bench-top instrumenst that are very easy to operate and can also automatically process up to 80 samples per hour.

This is a BSL 2 (Biosafety Level 2) facility. All users wishing to run samples should contact the Flow Cytometry Facility.


• Room 323 Burrill Hall
  This facility is equipped with a BD FACSCanto, 6 color flow cytometry analyzer for routine multicolor, multifunction analyses. This is bench-top machine has a more advanced analysis program but is still easy to use and can process up to 10,000 events per second.


BD FACSCanto 6 color cytometer


 

Default configuration for BD FACSCanto

Detector Array (Laser)	Longpass Dichroic Mirror	Bandpass Filter	Fluorochrome or Scatter Parameter
blue octagon (488-nm laser)	735 LP	780/60	PE-Cy7
	670 LP	655 LP	PerCP-Cy5.5, PerCP-CY5 PerCP, PI
	556 LP	585/42	PE
	502 LP	530/30	FITC, Alexa 488, GFP
	blank	488/10	SSC
	blank	blank	none
	blank	blank	none
	-	blank	none
	blank	Blank	none
	-	blank	none
red trigon (633-nm laser)	735 LP	780/60	APC-Cy7
	blank	660/20	APC, Alexa 647
	-	blank	none

Flow Cytometry Facility
Barbara Pilas, Ph.D - Director
231 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801
Phone: (217) 244-0559     FAX: (217) 244-0446      Email: pilas@uiuc.edu