Question: How do I start a project?
Answers:

Download a service request form the links:

Use Polyclonal Antibody Request Form for new polyclonal antibody generation
Use Monoclonal Antibody Request Form for new monoclonal antibody generation
Use Miscellaneous Services Request Form for monoclonal antibody reproduction with an existing hybridoma cell line or for other antibody services
Use Biacore Request Form for Biacore training and instrument usage

Fax completed request form to 217-244-0557 (our fax machine does not receive computer fax)

We will send you an email confirmation after we receive your request
We will inform you the date of first bleeding once your project is started.

Question: What are antigen requirements?
Answers:

Quantity : 2mg for a new antibody generation with one rabbit, one chicken, 5 mice or 3 rats. If animals are doubled, antigen required is doubled.
Purity : 90% or better. Contact us if an antigen is less than 90% pure. We might help you with further purification or accept an antigen as it is.
Concentration : minimal 1mg/ml. Accurate determination of antigen concentration is critical for a project success.
Buffer : Tris or phosphate buffer with a neutral pH (7-8) are acceptable. No detergents, reducing agents and imidazole are acceptable and should be dialyzed out. Most efficient dialysis method for our purpose is to use dialysis tubing with 1-2ml samples in 1L buffer stirring overnight at 4C. We can offer dialysis for you for $25. If an antigen does not mixed well with adjuvant due to insufficient dialysis (this happens very often!), $25 fee will be charged for further dialysis by us.
Sterilization : passing through 0.22um filter unit before concentration is determined.
Shipping: Dry ice or ice pack with overnight service.
Insoluble antigen : Insoluble antigen is acceptable if concentration is well measured. Contact us if you have problem determining concentration with an insoluble antigen.

Question: What dilution do I use to test an antibody sample? Answers :

Hybridoma medium can be tested without any dilution. A serum sample needs to be diluted 1:200 to 1:10,000 depending on which bleed and what animals are used. For rabbit serum, first bleed can be tested at 1:500 to 1:5000 dilutions. Later bleeds to be tested up to 1: 20,000. Mouse serum first bleeds can be tested at 1:200 to 1:1000. Later bleeds can be tested up to 1:5000. Chicken serum can be tested as rabbit serum. Chicken egg yolk and chicken serum contain the same concentration of antibody IgY.

Immunological Resource Center Liping Wang, Laboratory Supervisor 264D Burrill Hall, 407 South Goodwin Avenue, Urbana, IL 61801 Phone: (217) 244-0558 FAX: (217) 244-0557 Email: IRC@life.uiuc.edu
*Last edited:* July *2006*

Question: How do I start a project? Answers:

Question: What are antigen requirements? Answers:

Question: What dilution do I use to test an antibody sample? Answers :

Question: How do I start a project?
Answers:

Question: What are antigen requirements?
Answers: