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Immnological Resource Center
Service fees |
The service fees presented as follows are based on UIUC campus rates and subject to adjustment in July each year. Off-campus rate is additional 25.3 % on the basis of campus rate.
A. Polyclonal Antibody Generation
Animals (species)
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Minimum quantity of antigen
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*Antigen purity
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Testing samples
(Per animal)
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Final products
(Per animal) |
Time frame (weeks) |
Fees
( University of Illinois )
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| Rabbit
(NZW) | 1.5 mg for each animal |
90% |
2 bleeds (2 x10ml) |
70ml serum |
12-16 |
$596 (1animal)
$988 (2 animals)
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Chicken
(Farm chicken) |
1.5mg for each animal |
90% |
2 bleeds (2X500ul)
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*5-10 eggs |
12-16 |
$242 (1 animal)
$342 (2 animals) |
| Mouse
(Balb/c) |
1.5 mg for a project |
90% |
2 bleeds (2x100ul) |
300ul serum |
12-16 |
$457 (a cage of 5 mice) |
Rat (Lou/m)
Or Sprague Dawley |
1.5 mg for a project |
90% |
2 bleeds (2x100ul) |
2.5ml serum |
12-16 |
$457 (a cage of 3 rats) |
Notes:
- Extra serum/eggs are offered for an extra fee
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Off-campus clients should prepare an additional fund for a few packages of FedEx shipping. Currently, we charge $50 flat fee/package.
B. Monoclonal Antibody Generation with Mice or Rats
| Stages |
Services |
Time frame |
Products |
Fees ( Univ. of Illinois ) |
| I |
4-6 immunizations and 2-3 bleedings and ELISA tests |
9-16 weeks |
Testing serum from each alive animal |
$520 |
| II |
Lymphocyte fusion |
8-12 days |
N/A |
$358 |
| III |
Subculture and ELISA screening |
5 days |
2 ml hybridoma medium from selected culture |
$445 |
| IV |
Subcloning and ELISA screening and freezing |
30 days |
Hybridoma medium Frozen hybridoma cell lines |
$495 |
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Total production time |
4-6 months |
Total Price |
$1,818 |
Note:
- The fee covers only two fusions. An additional fusion cost $150.
- Off-campus clients should prepare an additional fund of for a few FedEx overnight shipping. We currently charge $50/package.
- Final products include up to 9 cell lines frozen in vials and 12ml of hybridoma medium from each cell line. Additional cell lines can be frozen at extra fees. For more medium production, please refer to the following medium production services.
C. Monoclonal Antibody Reproduction (medium) with Hybridoma Cells
| Methods |
Products |
Antibody concentration |
Time frame |
Fees ( Univ. of Illinois ) |
Conventional static flask culture |
Up to 30ml |
0.02 -0.1mg/ml |
4 –10 days |
$1.5/ml |
30ml-1000ml |
0.02 -0.1mg/ml |
10-25 days |
$0.45/ml |
Integra CL350 bioreactor |
40ml
Bioreactor medium |
~1mg/ml |
8-9 weeks |
$581 |
30ml
Pre-bioreactor medium |
0.02-0.1mg/ml |
*Mouse ascites |
4ml per mouse |
1-10mg/ml |
7 weeks |
$314 (5 mice)
$520 (10 mice)
$700 (15 mice)
$880 (20 mice) |
Notes :
- Ascites production is only allowed (by campus authority) if a hybridoma cell line is failed to grow in Integra Bioreactor.
- Some fetal bovine serum (FBS) is from culture medium and contained in bioreactor antibody products. If FBS is a problem for product application, serum-free medium can be tried for a quoted price.
D. Antibody Purification
Service |
Application |
Starting volume |
Time Frame |
Fee
( Univ. of Illinois) |
Delipidation |
Remove lipid and lipoproteins |
Various |
1 day |
$0.68/ml |
Ammonium sulfate precipitation |
Partial purify antibody |
Various |
5 days |
$0.6/ml |
Concentrating antibody |
Various |
3 days |
$0.6/ml |
Dialysis |
Remove small molecules or buffer exchange |
3- 15 ml |
3 days |
$30 |
Antigen affinity purification |
Purification for antigen specific antibody |
First 25mg |
1 week |
$279 |
Additional 25mg |
2 days |
$111 |
Chicken antibody purification |
IgY extraction from egg yolk |
1ml yolk |
1 day |
$35 |
A whole yolk (~36ml) |
1 day |
$62 |
Hydroxyapatite
Column |
IgG purification |
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Set up, 1 week |
$250 |
Up to 50mg |
1 week |
$60 |
Protein A
Protein G
Protein L |
IgG and IgM purification on quotation |
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E. Miscellaneous Antibody Services
| Service |
Fee |
Quantity |
ELISA (serum, medium) |
$50 |
One 96-well plate |
Antibody concentration assay (serum, medium, ascites ) |
$65 |
Up to 2 samples |
Antibody cross-reactivity assay |
$70 |
2 x 96-well plates |
Antibody isotyping |
$24 |
One cell line |
Antigen isolation from SDS gel |
$25 |
1 sample |
Peptide conjugation to carrier (KLH, BSA or OvA) |
$60 |
One peptide to one carrier |
Protein Biotinylation |
$50 |
One protein (up to 10mg) |
F. Biacore 3000 Services
Service |
Fee |
Instrument individual training |
$100 |
Instrument usage hourly rate
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$35
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For Biacore training, consultation and services, please contact lab supervisor Liping Wang at 217-244-0558 or lpwang@life.uiuc.edu. For Biacore technical information and products, please visit http://www.biacore.com.
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Immunological Resource Center
Liping Wang, Laboratory Supervisor
264D Burrill Hall, 407 South Goodwin Avenue, Urbana, IL 61801
Phone: (217) 244-0558 FAX: (217) 244-0557 Email: IRC@life.uiuc.edu
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| Last edited: 07, June 2007
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