Research Topics
Department of Animal Sciences
Ph.D. (1986) Colorado State University
Research Topics
Research Interests
The research interests of my laboratory focus on understanding the molecular and cellular mechanisms involved in the development differentiated function in ovarian and embryonic cells. To study such processes we utilize swine cattle, and mice as model systems. My group is studying embryo development and gene expression using transgenic animal technology. We are involved in studies which are examining; 1) methods to increase the efficiency of production of transgenic animals, 2) vector systems which would allow tissue specific expression of 'foreign' genes in the transgenic genome and 3) development and characterization of gene expression in embryonic stem cells. Currently, I am studying the molecular and cellular mechanisms of differentiation during early embryonic development. This has allowed development of an elegant system for embryonic differentiation using embryonic stem (ES) cells derived from swine embryos. We have used this system to produce the first chimeric pigs which provides a potentially more efficient method to produce transgenic swine. We are examining signals which initiate differentiation in ES cells and characterization of the first genes that are expressed during this differentiation. In addition, we are studying the fate (fate mapping) of ES cells as they are incorporated into differentiated tissues of the embryo and fetus. Studies are being conducted to determine the feasibility of utilizing swine ES cell nuclei in nuclear transfer or cloning technologies. Finally, vector systems which would allow tissue specific expression of 'foreign' genes in the transgenic genome, specifically into reproductive tissues including milk, are being studied.
Key Words
Recombinant DNA, Introduction of DNA into Animal Cells, Gene
Expression Systems, DNA-Protein Interactions, Genome Organization/RFLP Mapping,
Hormones, Animal Cell and/or Tissue Culture, Transgenic Animals, Membrane Structure
and Function, Protein Purification and Characterization, Protein Processing
and Secretion, Active Sites and Receptors
Current Research Funding USDA,
DARPA, C-FAR